identification of campylobacter jejuni and campylobacter coli from diarrheic samples using pcr
نویسندگان
چکیده
background: campylobacter jejune and c. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. in this study identification of campylobacter jejuni and campylobacter coli from samples using pcr was explored. methods: detection was performed using diarrheal samples collected 8 from 117 children. the genomic dna of samples was extracted by phenol-chloroform method. all dna extracts were examined for the presence of c.jejuni and c.coli species based on pcr method. results: of 117 diarrheal samples, 35 (29.9%) were found positive for 10 campylobacter spp using pcr. conclusions: the results of this study showed that pcr is effective for rapidly screening stool samples for campylobacter spp, due to its high sensitivity and specificity.
منابع مشابه
PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.
Three sets of primers were designed for PCR detection and differentiation of Campylobacter jejuni and Campylobacter coli. The first PCR assay was designed to coidentify C. jejuni and C. coli based on their 16S rRNA gene sequences. The second PCR assay, based on the hippuricase gene sequence, identified all tested reference strains of C. jejuni and also strains of that species which lack detecta...
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Campylobacter species, primarily Campylobacter jejuni and Campylobacter coli, are regarded as a major cause of human gastrointestinal disease, commonly acquired by eating undercooked chicken. We describe a PCR-ELISA for the detection of Campylobacter species and the discrimination of C. jejuni and C. coli in poultry samples. The PCR assay targets the 16S/23S ribosomal RNA intergenic spacer regi...
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Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals’ food and water were collected from G. B. Pant High Altitude Zoo, Nainital, ...
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Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, conventional culture and multiplex PCR methods were compared for the detection of C. jejuni isolated from poultry carcasses. A total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...
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Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolat...
متن کاملSerotyping and Identification of Campylobacter Jejuni and Campylobacter Coli Strains of Human and Animal Origin Using the Pcr Method
Steinhauserová I . , K. Foj t íková: Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method. Acta Vet. Brno 1999, 68: 149–154. A total number of 178 strains of Campylobacter spp. from human patients suffering from diarrhea and from the intestinal content of slaughtered pigs and poultry was isolated during years 1996 t...
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عنوان ژورنال:
international journal of health studiesجلد ۲، شماره ۲، صفحات ۰-۰
کلمات کلیدی
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